我国农作物绿色食品的生产瓶颈和生产前景

生产农作物绿色食品是农业的发展方向，也是可持续发展的必然要求，是世界范围都要引起 重视的普遍课题，我国更需迫切重视。使用一般的无公害杀虫剂是不能生产出绿色食品的，而目前病 虫害绿色防控技术还不成熟，现在可用于绿色食品生产的高效无毒的杀虫剂在我国已首先研究出来 了，当前缺少的仅是农产品市场完备的农药残留检测机制，解决这个瓶颈问题可给绿色食品创造存在 的空间，从而实现农业绿色产业大发展，这一天的到来已为期不远。     

乡村振兴背景下贵州食用菌精准扶贫产业的现状与路径创新

为促进贵州省食用菌基地大幅度进步和食用菌产业的发展,针对贵州省食用菌基地现阶段发展详情,可能存在的问题,并结合国家现阶段发展政策,对贵州省食用菌基地发展提供科学、合理、有效的建议。     

MicroRNA-9-5p mediates sympathetic overactivity by targeting KCNN3 in paraventricular nucleus of rats with T2D

AIM To investigate whether microRNA-9-5p （miR-9-5p） mediates sympathetic overactivity by targeting KCNN3 （potassium intermediate/small conductance calcium-activated channel, subfamily N, member 3） gene,which encoded small-conductance calcium-activated potassium channel 3 （SK3） protein, in paraventricular nucleus （PVN） of rats with type 2 diabetes mellitus （T2D）. METHODS A rat model of T2D was established by high-fat diet combined with intraperitoneal injection of 30 mg/kg streptozotocin. The levels of miR-9-5p and KCNN3 mRNA in PVN were detected by real-time PCR. The relationship between KCNN3 and miR-9-5p was predicted by TargetScan. Recombinant adeno-associated virus （rAAV）-miR-9-5p or KCNN3 were bilaterally microinjected into the PVN to observe the changes in plasma glucose levels and sympathetic drive indicators. The number of FosB and SK3 positive cells was measured by immunofluorescence staining. The protein expression of SK3 was determined by Western blot. The relationship between KCNN3 and miR-9-5p were confirmed by cell transfection and dual-luciferase reporter assay. RESULTS Compared with the rats in diabetes control （DC） group, the blood glucose, sympathetic drive indexes and the level of miR-9-5p in PVN were significantly increased, while the SK3 expression in PVN was obviously reduced in the diabetes mellitus （DM） rats. After microinjecion of rAAV-miR-9-5p in PVN, the sympathetic drive indexes, blood glucose, and the number of FosB-positive cells were increased significantly, but the SK3 protein expression was significantly reduced （P<0.05）. However, up-regulation of KCNN3 in PVN had the opposite effect. These responses were obviously enhanced in DM rats compared with DC rats. The results of cell transfection and dual-luciferase reporter assay demonstrated that miR-9-5p bound to the 3’-UTR of KCNN3 and inhibit its expression. CONCLUSION miR-9-5p was up-regulated in PVN of the rats with T2D, and it may mediate sympathoexcitation by targeting KCNN3.     

酵母基因表达调控关系的构建及其统计特性分析

[目的]从复杂网络的角度研究酵母细胞周期调控网络的统计特性。[方法]首先对酵母的细胞周期基因表达数据采用皮尔逊相关性度量,计算基因间的关联矩阵,即建立简单的基因调控网络;同时为了能够得到在宏观角度对大规模的基因调控有一个全貌的了解,进一步分析所建立调控网络的统计特性,通过计算所建立网络的统计特性：即计算网络的聚类系数、平均路径长度和网络的度分布。[结论]发现所建立的网络具有无标度和小世界特性,即所建立的酵母基因调控网络属于复杂网络,这为进一步深入研究基因调控网络的统计学和动力学特性打下了基础。     

东北农牧交错带小尾寒羊集群放牧系统中钙、磷元素营养状况分析

对我国东北农牧交错带内小尾寒羊集群放牧生态系统中5、7、9月钙、磷元素的营养状况进行了分析。结果表明:牧地土壤中钙、磷元素的总平均含量分别为1.46%、178.88μg/g;小尾寒羊2种主食牧草中钙、磷元素的总平均含量分别为0.39%、256.26μg/g;小尾寒羊羊毛中钙元素的总平均含量为2111.45μg/g;小尾寒羊羊血中钙、磷元素的总平均含量分别为69.65μg/g、10.21μg/g;小尾寒羊羊骨中钙、磷元素的总平均含量分别为4.44%、2.45%。据此认为,在该交错带集群放牧的小尾寒羊不会发生钙缺乏症或中毒病,但可能发生磷缺乏症。     

清末呼兰地区移民与农业开发

呼兰是清政府在东北最早开禁的地区。1860～1903年，清朝先后开放了蒙古尔山、通肯等地区，关内移民开始大量涌入呼兰地区．土地开发规模迅速扩大，粮食产量大幅度提高，农产品加工业崛起，农产商品率迅速提高，促进了农业的近代化。     

金代农业科学发展探讨

从耕作技术、水利兴修和农田灌溉3方面介绍了金代农业科学的成就，为人们了解金代农业生产方式提供参考。     

高山红景天愈伤组织的超低温保存

高山红景天叶片在附加6-BA 2 mg·L-1和NAA 0.5 mg·L-1的改良MS培养基中诱导出愈伤组织,将愈伤组织放入10%DMSO 0.5 mol·L-1山梨醇的冰冻保护剂中,4℃冰箱中放置8 h,然后以1℃·min-1的降温速度降至-40℃,停留2 h后投入液氮保存,在40℃水浴中化冻.冻存后的愈伤组织用液体培养基MS 6-BA 2 mg·L-1 NAA 0.5 mg·L-1反复冲洗后,转入固体培养基中进行暗培养,2周后转入光照(2 000 1x)培养.5周后观察到高山红景天愈伤组织呈鲜绿色,长势较为旺盛,愈伤组织存活率达到75.64%.冻存后的愈伤组织可诱导成苗.     

Dynamics of Zn between soil and plant in Northeast Leymus chinensis grassland

A study was conducted to determine the dynamic of Zn content between soil and plant in the natural protection zone of Leymus chinensis grassland in Changling County (44°30′–44°45′N; 123°31′–124°10′E), Jilin Province, China. Results showed that the total Zn content was lower, available Zn content had a moderate level in the soil, and the plants was not lack of Zn. During the growing season, content of total Zn and available Zn in soil showed a down-trend distribution along the soil profile. Content of total Zn had a significantly positive correlation with that of the organic matter, but it was negatively correlated to soil pH. Monthly dynamic of the average content of total Zn showed a “V” type curve in the growing season from May to August, and July was the nadir. The trend of the average content of available Zn was similar to the content of total Zn, but was down after August; Zn content variation in the organs and litter of L. chinensis was great, with the order of root>rhizome >leaf>stem>litter. The ratio of available Zn content in A layer versus B layer was more than 2 times that of the total Zn, which indicated that the soil of A layer had higher enrichment capacity of available Zn. The enrichment of Zn in the root of L. chinensis was 44.17 times as that in the soil. The absorbing intensity of root had a significantly negative correlation with the activity of Zn in the soil (r=−0.8800, p<0.01). Biography: ZHOU Xiao-mei (1966–), female, Ph.D., associate professor of Jilin Normal University, Siping 136000, P.R. China.     

吉林架树台湖湿地公园退化湿地修复对策及建议

本文介绍了吉林架树台湖湿地公园的基本概况和湿地退化状况,并从湿地生态补水、渠系改造和修复、水域清淤、珍稀水鸟及栖息地生境保护与修复等方面提出了湿地修复的对策及建议。